Hb = Hemoglobin Ht = Hematocrit MCHC = Mean Corpuscular Hemoglobin Concentration Alb = Albumin AFP = Alpha-feto protein

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1 List of abbreviations LUMC = Leiden University Medical Center IgG = Immunoglobulin G Rh D = Rhesus D (antibody) Delta OD450 = Delta Optical Density 450 (spectrophotometric measurement) CMV = Cytomegalovirus IUT = Intrauterine transfusion ADCC = Antibody-Dependent Cell-mediated Cytotoxicity assay BAR = Bilirubin/ albumin ratio FPV = Feto-placental blood volume RCV = Red cell volume Hb = Hemoglobin Ht = Hematocrit MCHC = Mean Corpuscular Hemoglobin Concentration Alb = Albumin AFP = Alpha-feto protein SD = Standard deviation SE = Standard error 95%CI = 95% Confidence interval ROC curve = Receiver operating characteristics curve p = probability R 2 = Coefficient of determination L = liter dl = deciliter ml = milliliter kg = kilogram g = gram mg = milligram mmol = millimol nm = nanometer kd = kilodalton 9 143

2 Chapter 9 144

3 Authors and affiliations From the department of Obstetrics, division of Fetal Medicine, Leiden University Medical Center, Leiden, The Netherlands: Esther Sikkel, Suzanne A. Pasman, Dick Oepkes, Humphrey H.H. Kanhai, Frank P.H.A. Vandenbussche, Robertjan H. Meerman, Colinda P. Bil- van den Brink, Metteke A. Kamping, Phebe N. Adama van Scheltema From the department of Medical Statistics, Leiden University Medical Center, Leiden, The Netherlands: Saskia LeCessie From the department of Clinical Chemistry, Leiden University Medical Center, Leiden, The Netherlands: Freek W.C. Roelandse From the department of Pathology, Leiden University Medical Center, Leiden, The Netherlands: Emile de Heer 9 145

4 Total blood volume is maintained in nonhydropic fetuses with severe hemolytic anemia Pasman SA, Bil-Van den Brink CP, Kamping MA, Adama van Scheltema PN, Oepkes D, Vandenbussche FP. Fetal Diagn Ther. 2009; 26(1):10-5. Epub 2009 Oct 10. Quantification of feto-fetal transfusion rate through a single placental arterio-venous anastomosis in a monochorionic twin pregnancy Lopriore E, Van den Wijngaard JP, Pasman SA, Oepkes D, Walther FJ, Van Gemert MJ, Vandenbussche FP. Placenta Mar; 30(3): Epub 2008 Dec 24. Arterio-venous flow between monochorionic twins determined during intra-uterine transfusion. Nonlinear decay of adult red blood cells Van Gemert MJ, Pasman SA, Van den Wijngaard JP, Lopriore E, Vandenbussche FP. Phys Med Biol Jul 7; 53(13):L11-4. Epub 2008 Jun 17. Bilirubin/albumin ratios in fetal blood and in amniotic fluid in rhesus immunization Pasman SA, Sikkel E, Le Cessie S, Oepkes D, Roelandse FW, Vandenbussche FP. Obstet Gynecol May; 111(5): Arterio-venous flow between monochorionic twins determined during intra-uterine transfusion Van Gemert MJ, van den Wijngaard JP, Lopriore E, Pasman SA, Vandenbussche FP. Phys Med Biol Apr 7; 53(7):N Epub 2008 Mar 18. Hypoalbuminemia: a cause of fetal hydrops? Pasman SA, Meerman RH, Vandenbussche FP, Oepkes D. Am J Obstet Gynecol Apr; 194(4): On the origin of amniotic fluid bilirubin Sikkel E, Pasman SA, Oepkes D, Kanhai HH, Vandenbussche FP. Placenta May; 25(5):

5 Publications A new method to determine the feto-placental volume based on dilution of fetal haemoglobin and an estimation of plasma fluid loss after intrauterine intravascular transfusion Hoogeveen M, Meerman RH, Pasman SA, Egberts J. BJOG Oct; 109(10):

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7 Curriculum vitae Suzanne A. Pasman werd op 10 augustus 1977 geboren te Nieuwegein. Zij groeide op in een gezin met één zus en twee broers. Na op diverse plekken te hebben gewoond, onder andere in Camberley te Engeland, bracht zij het grootste deel van haar jeugd door in Boxmeer. In 1995 behaalde zij er aan het Elzendaal college het Gymnasium diploma. In 1996 behaalde zij de propedeuse Biomedische Wetenschappen te Leiden. Daarna vervolgde zij met de studie Geneeskunde. In dit jaar was zij tevens voorzitter van de gezelligheidsvereniging van de faculteit: het Leids Medisch Dispuut Forestus (250 leden). Voor en na deze bestuurstijd was zij tevens betrokken bij diverse serieuze en minder serieuze commissies binnen de faculteit. Op 9 april 1998 werd zoon Myrddin geboren. Vanaf dat moment studeerden zij en partner Sebastian Peters afwisselend. Zo doorliep zij in 2000 het eerste jaar van de co-schappen en in 2003 het tweede jaar. Op 3 februari 2002 vond de geboorte plaats van een tweede zoon: Thorquato. Tevens werd in deze periode het eerste contact gelegd met Dr. Frank Vandenbussche van de afdeling Verloskunde in het LUMC. Er werd in deeltijd gestart met het onderzoek dat uiteindelijk tot dit proefschrift heeft geleid. In februari 2004 trad zij in dienst als arts-echoscopist bij de sectie prenatale diagnostiek en behandeling van de afdeling Verloskunde in het LUMC, tot vorig jaar onder leiding van Prof. Dr. Humphrey Kanhai. Daar is zij tot op heden werkzaam, inmiddels als arts prenatale geneeskunde onder leiding van Dr. Dick Oepkes en Prof. Dr. Jan van Lith. Binnen de foetale geneeskunde ontwikkelde zij niet alleen een voorliefde voor de patiëntenzorg en het onderzoek maar ook voor onderwijs

8 Chapter 9 150

9 Dankwoord Bloed krijg én geef je met je hart! Voor alle hulp die ik gekregen heb in de afgelopen jaren, wil ik graag mijn dank aan jullie geven uit het hart: aan alle zwangeren, alle foetussen, alle bloeddonoren aan iedereen die mijn werk en onderzoek ondersteund heeft op de poli, het secretariaat en op de afdeling verloskunde aan iedereen die mijn werk en onderzoek ondersteund heeft op het Verloslab, het CKCL en de Immunohematologie aan de student-onderzoekers met wie ik heb mogen samenwerken aan alle mede-auteurs en al mijn leermeesters aan mijn collega s, die mij onder meer de mogelijkheid hebben gegeven om dit werk te voltooien aan mijn vrienden, familie en schoonfamilie aan Myrddin en Thorquato (en iedereen die bij heeft gedragen aan hun opvang) Sebastian, without you this would all still be nothing more than a figment of my imagination 9 151

10 152

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