Kleuren met: Prisma & Coverslipper HQ plus. Door: Jorrit van het Bolscher Aplication specialist Sakura

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1 Kleuren met: Prisma & Coverslipper HQ plus Door: Jorrit van het Bolscher Aplication specialist Sakura

2 Agenda Update Prisma & Coverslipper HQ plus Kleuringen (H&E) Bij de gebruiker Wat als: De Kleuring niet mooi of constant is..

3 Prisma Throughput & speed Flexibility Proven reliability 660 slides/hour Routine & Special Continuously loading and 11 protocols simultaneously running! 3

4 Introducing: Prisma & Coverslipper HQplus

5 Introducing: Prisma & Coverslipper HQ plus HQ plus New & improved H&E formula in combination with optimized & validated protocols Consistent high-quality staining up to slides Integrated barcode reader for efficient slide tracking Fastest multi-stainer and coverslipper

6 Prisma HQ Staining New and improved H&E staining formula Proven consistent high-quality staining up to slides Packaging for ease of use and increased safety Efficiency and time saving Integration by system matching volumes Flexibility to optimize staining results

7 Results H&E Staining, optimized formula Placenta Colon Prostate Slide 1 Slide Slide Slide

8 HQ staining: an example in more detail Examples: Placenta 3 tones of pink (eosin) staining Erytrocytes, red blood cells Connective tissue Connective tissue Haematoxyline Crisp blue/purple nuclear detail 8

9 Ready to Use (RTU) H&E Staining Kits H&E Staining Kit 270 ml Item 8640 for Medium Solution reservoirs H&E Staining Kit 750 ml Item 8641 for Large Solution reservoirs 9

10 Prisma Coverslipper Plus Film Glas g2 10

11 Plus: Barcode reader integration Barcode reader to support track and trace capabilities Barcode reader sends a file in data form via USB Results can be sent to an LI(M)S or stand alone computer Results are connected to Track and Trace System to verify finished Coverslipping 11

12 Plus: Barcode reader integration Benefits Feedback when slides are ready for reporting Reduction of recording errors Free up resources: manual scanning of the slides Easier tracing of root causes 12

Summary: Prisma & Coverslipper HQ plus Optimized Staining results Ready-to-use H&E Staining kits Documentation and specialized support Easy of use and increased safety Consistent Staining result

13 Summary: Prisma & Coverslipper HQ plus Optimized Staining results Ready-to-use H&E Staining kits Documentation and specialized support Easy of use and increased safety Consistent Staining result Validated Sakura protocols Clear cut off point to replace reagents Integrated Slide Barcode Reading Track when the slides are ready Aid with tracing root cause Free resources for more value adding work Maximized productivity Fastest system in the market with highest throughput 13

14 Voorbeelden Gebruik Prisma 1 Klant voorbeeld HE gebruik gekoppeld aan FILM Afdekmachine 2 Klant voorbeeld HE en PAP Kleuring gekoppeld aan FILM Afdekmachine 3 Klant voorbeeld HE en Speciaal Kleuringen gekoppeld aan FILM Afdekmachine

15 Voorbeeld Gebruik Prisma : 1: HE gebruik gekoppeld aan FILM Afdekmachine

16 Voorbeeld Gebruik Prisma : 1: HE gebruik gekoppeld aan FILM Afdekmachine Program Name:HE+OVEN Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:15:00 ** OFF 3 21 Xylene 00:02:00 ** OFF 4 20 Xylene 00:02:00 ** OFF 5 19 Xylene 00:02:00 ** OFF 6 18 Alcohol 100% 00:02:00 ** OFF 7 17 Alcohol 100% 00:02:00 ** OFF 8 9 Alcohol 100% 00:02:00 ** OFF 9 10 Alcohol 90% 00:02:00 ** OFF 10 1 Alcohol 70% 00:02:00 ** OFF 11 W* Wash Station 00:02:00 ** OFF 12 2 Hematoxylin(Mayers) 00:03:00 20% EW 13 3 Hematoxylin(Mayers) 00:03:00 20% ON 14 W* Wash Station 00:05:00 20% EW Ammonia Water 00:00:27 == ON 16 W* Wash Station 00:01:00 50% OFF Erytrosine 00:01:30 20% ON 18 W* Wash Station 00:01:00 20% EW Alcohol 80% 00:01:00 20% OFF Alcohol 100% 00:01:00 ** OFF Alcohol 100% 00:01:00 ** OFF Alcohol 100% 00:01:00 ** OFF Alcohol 100% 00:01:00 ** OFF Xylene 00:02:00 ** OFF Xylene 00:02:00 ** OFF 26 E* End Station --:--:-- Total 26 Steps

17 Voorbeeld Gebruik Prisma : 2: HE en PAP kleuring met FILM Afdekmachine Program Name: HE Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 17 Xyleen 00:01:30 ** ON 4 9 Xyleen 00:01:30 ** OFF 5 1 Iso :01:30 ** OFF 6 2 Methanol 00:01:30 ** OFF 7 W* Wash Station 00:01:30 ** OFF 8 15 Hematoxyline 00:03:00 20% ON 9 W* Wash Station 00:01:30 20% OFF 10 3 HCL 00:00:30 20% ON 11 W* Wash Station 00:00:30 20% OFF Li Carbonaat 00:00:30 20% ON 13 W* Wash Station 00:00:30 20% OFF Erythrosine 00:00:30 20% ON 15 W* Wash Station 00:00:30 20% OFF Iso :01:30 ** ON Iso :01:30 ** OFF Xyleen 00:01:30 ** ON Xyleen 00:01:30 ** OFF 20 E* End Station --:--:-- Program Name: PAP Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 W* Wash Station 00:03:00 ** OFF 3 15 Hematoxyline 00:02:00 ** ON 4 W* Wash Station 00:02:00 ** ON 5 28 Iso 70 00:01:30 ** OFF 6 23 Orange G 00:01:00 20% ON 7 22 Iso 70 00:02:00 ** OFF 8 21 Iso 70 00:02:00 ** OFF 9 27 Light Green 00:02:00 ** ON Iso 70 00:02:00 ** ON Iso :02:00 ** OFF Xyleen 00:01:30 ** ON Xyleen 00:01:30 ** ON 14 E* End Station --:--:-- Overige programma s

18 Voorbeeld Gebruik Prisma : 3: HE en Speciaal kleuringen met FILM Afdekmachine

19 Program Name:HE Voorbeeld Gebruik Prisma : 3: HE en Speciaal kleuringen met FILM Afdekmachine Program Name:PAS Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 44 Xylene 00:02:00 ** OFF 4 43 Xylene 00:02:00 ** OFF 5 41 Alcohol 100% 00:02:00 ** OFF 6 42 Alcohol 100% 00:02:00 ** OFF 7 40 Alcohol 70% 00:02:00 ** OFF 8 W* Wash Station 00:02:00 ** OFF 9 39 A DEST 00:02:00 ** OFF Haematox 00:02:00 ** OFF 11 W* Wash Station 00:03:00 ** OFF 12 6 Alcohol 70% 00:02:00 ** OFF 13 1 Alcohol 100% 00:02:00 ** OFF 14 2 Eosin Y 00:01:30 ** OFF Alcohol 100% 00:02:00 ** OFF Alcohol 100% 00:02:00 ** OFF Xylene 00:02:00 ** OFF Xylene 00:03:00 ** OFF 19 E* End Station --:--:-- Total 19 Steps Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 28 Xylene 00:01:00 ** OFF 4 27 Xylene 00:01:00 ** OFF 5 25 Alcohol 100% 00:01:00 ** OFF 6 26 Alcohol 100% 00:01:00 ** OFF 7 24 Alcohol 70% 00:01:00 ** OFF 8 23 A DEST 00:01:00 ** OFF 9 20 Perj 1% 00:10:00 ** OFF 10 W* Wash Station 00:01:00 ** OFF A DEST 00:01:00 ** OFF SCHIFF`S 00:20:00 ** OFF 13 W* Wash Station 00:05:00 ** OFF Hematoxylin 00:01:00 ** OFF 15 W* Wash Station 00:03:00 ** OFF Alcohol 100% 00:01:00 ** OFF Alcohol 100% 00:02:00 ** OFF Xylene 00:01:00 ** OFF Xylene 00:02:00 ** OFF 20 E* End Station --:--:-- Total 20 Steps

20 Voorbeeld Gebruik Prisma : 3: HE en Speciaal kleuringen met FILM Afdekmachine Program Name:EVG Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 28 Xylene 00:01:00 ** OFF 4 27 Xylene 00:01:00 ** OFF 5 25 Alcohol 100% 00:01:00 ** OFF 6 26 Alcohol 100% 00:01:00 ** OFF 7 24 Alcohol 70% 00:01:00 ** OFF 8 23 A DEST 00:01:00 ** OFF 9 3 Elastica 00:30:00 ** OFF 10 W* Wash Station 00:02:00 ** OFF 11 4 Van Gieson's 00:10:00 ** OFF Alcohol 100% 00:01:00 ** OFF Alcohol 100% 00:01:00 ** OFF Xylene 00:01:00 ** OFF Xylene 00:02:00 ** OFF 16 E* End Station --:--:-- Total 16 Steps Program Name:ALCIAN BLUE Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 28 Xylene 00:02:00 ** OFF 4 27 Xylene 00:02:00 ** OFF 5 25 Alcohol 100% 00:02:00 ** OFF 6 26 Alcohol 100% 00:02:00 ** OFF 7 24 Alcohol 70% 00:02:00 ** OFF 8 23 A DEST 00:02:00 ** OFF 9 36 Alcian Blue 00:30:00 ** OFF 10 W* Wash Station 00:01:00 ** OFF Kernecht rood 00:05:00 ** OFF 12 W* Wash Station 00:01:00 ** OFF Alcohol 100% 00:02:00 ** OFF Alcohol 100% 00:02:00 ** OFF Xylene 00:02:00 ** OFF Xylene 00:03:00 ** OFF 17 E* End Station --:--:-- Total 17 Steps

21 Wat als: Application Bulletin H&E Staining kits V-1.pdf

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28 Bedankt voor jullie aandacht!

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